Paolo Swuec, PhD

 

Postdoctoral Researcher

paolo.swuec@unimi.it

 

 

Publications (May 2017):

 

- Marcin J *, Swuec P. *, Kasprzak J, Renault L, Dobrychłop M, Gaur V, Bujnicki J, Costa A and Nowotny N. (2017) The mechanism of DNA damage verification in bacterial nucleotide excision repair. (manuscript submitted)  * Authors contributed equally to this work

 

- Ballandras-Colas, A.*, Maskell, D.P.*, Serrao, E., Locke, J., Swuec, P. , Jonsson, S.R., Kotecha, A., Cook, N.J., Pye, V.E., Taylor, I.A., Andresdottir, V., Engelman, A.N., Costa, A. and Cherepanov, P. (2017) A supramolecular assembly mediates lentiviral DNA integration. Science, doi: 10.1126/science.aah7002 * Authors contributed equally to this work

 

- Swuec, P. , and Costa, A. (2016). DNA replication and inter-strand crosslink repair: Symmetric activation of dimeric nanomachines? Biophys Chem, doi: 10.1016/j.bpc.2016.11.001.

 

- Swuec, P. , Renault, L., Borg, A., Shah, F., Murphy, V.J., van Twest, S., Snijders, B., Deans, A.J., and Costa, A. (2016). The FA Core Complex Contains a Homo-dimeric Catalytic Module for the Symmetric Mono-ubiquitination of FANCI-FANCD2. Cell Rep, doi: 10.1016/j.celrep.2016.11.013.

 

- van Twest, S., Murphy, V.J., Hodson, C., Tan, W., Swuec, P. , O'Rourke, J.J., Heierhorst, J., Crismani, W., and Deans, A.J. (2016). Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway. Molecular Cell, doi: 10.1016/j.molcel.2016.11.005.

 

- Pike, A.C.*, Gomathinayagam, S.*, Swuec, P. *, Berti, M., Zhang, Y., Schnecke, C., Marino, F., von Delft, F., Renault, L., Costa, A., et al. (2015). Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures. Proc Natl Acad Sci USA, doi: 10.1073/pnas.1417594112 4286-4291.  * Authors contributed equally to this work

 

- Costa, A., Renault, L., Swuec, P. , Petojevic, T., Pesavento, J.J., Ilves, I., MacLellan-Gibson, K., Fleck, R.A., Botchan, M.R., and Berger, J.M. (2014). DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome. eLife, doi: 10.7554/eLife.03273.

 

- Swuec, P. , and Costa, A. (2014). Molecular mechanism of double Holliday junction dissolution. Cell Biosci, doi: 10.1186/2045-3701-4-36.

 

- Coulthard, R., Deans, A.J., Swuec, P. , Bowles, M., Costa, A., West, S.C., and McDonald, N.Q. (2013). Architecture and DNA recognition elements of the Fanconi anemia FANCM-FAAP24 complex. Structure, doi: 10.1016/j.str.2013.07.006.

 

- Swuec, P. , and Barlow, D.J. (2012). Prediction of inhibitory activities of Hsp90 inhibitors. Bioorg Med Chem, doi: 10.1016/j.bmc.2011.10.069.

 

Techniques Expertise (May 2017):

 

- Protein Expression and Purification: SDS-PAGE, western blotting, bioinformatics-based construct design, high-throughput cloning techniques (Gateway, In-Fusion, LIC, traditional), bacterial protein expression, baculovirus protein expression, AKTA HLPC Systems (Pure, Ettan, Micro), chromatographic methods (IMAC/SEC/IEX), affinity tag purification (FLAG, StrepII, MBP, SUMO).

 

- Protein-Protein\Protein-DNA Interactions: Multi-Angle Light Scattering, Fluorescence Polarization, Octet Red 96, ITC, MST, Yeast-2-Hybrid, Peptide Array.

 

- EM Sample preparation: carbon evaporation and coating, negative stain EM (uranyl acetate, uranyl formate, Nano-W), manual and automated cryo-EM grid plunge freezing (FEI Vitrobot Mark III\IV, Gatan CP3), sample stabilization by GraFix (ultracentrifugation in glycerol and glutaraldehyde gradient), EM grid functionalization with streptavidin 2D-crystals, Immunolabelling.

 

- EM Data Collection: FEI Talos Arctica, FEI Tecnai Spirit Bio-Twin, FEI Tecnai Twin, FEI Tecnai F20, FEI Tecnai T20, JEOL JEM-2100.

 

Software Expertise (May 2017):

 

- Single-particle reconstitution: RELION (1.2 - 2.1), EMAN(1.9/2.0/2.1), CryoSPARC, IMAGIC, Tigris, IMOD, Xmipp, SPARX, SIMPLE.

 

- Others: Scaffold 4, Serialcloner, SeqMan Pro, SeqBuilder, PyMOL, Chimera, ImageJ, Windows, Linux, OS X, Microsoft Office, Adobe Illustrator, Adobe Photoshop, Adobe Lightroom, EndNote, Papers 2.